巢湖学院学报 ›› 2019, Vol. 21 ›› Issue (6): 95-101.doi: 10.12152/j.issn.1672-2868.2019.06.014

• 化学与材料科学 • 上一篇    下一篇

白芨多糖的超声辅助提取工艺及抗氧化活性研究

巢湖学院化学与材料工程学院,安徽 巢湖 238000   

  1. 巢湖学院化学与材料工程学院
  • 收稿日期:2019-07-08 出版日期:2019-11-25 发布日期:2020-03-13
  • 通讯作者: 吴梦晴(1993-),女,安徽安庆人,巢湖学院化学与材料工程学院助教,主要从事药物制剂、天然药物的制备与修 饰研究。
  • 作者简介:吴梦晴(1993-),女,安徽安庆人,巢湖学院化学与材料工程学院助教,主要从事药物制剂、天然药物的制备与修 饰研究。
  • 基金资助:
    安徽省自然科学基金青年项目(项目编号:1908085QC123);安徽省教育厅高校学科(专业)拔尖人才学术资助项目(项目编号:gxbjZD37);巢湖学院校级科学研究一般项目(项目编号:XLY-201911)

Ultrasound-assisted Extraction Process and Antioxidant Activity of Bletilla Striata Polysaccharide

School of Chemistry and Material Engineering, Chaohu University, Chaohu Anhui 238000   

  1. School of Chemistry and Material Engineering, Chaohu University
  • Received:2019-07-08 Online:2019-11-25 Published:2020-03-13
  • Contact: WU Meng-qing:School of Chemistry and Material Engineering, Chaohu University
  • About author:WU Meng-qing:School of Chemistry and Material Engineering, Chaohu University
  • Supported by:
    1908085QC123;gxbjZD37;XLY-201911

摘要: 白芨多糖在医药领域及食品科学方面有诸多应用,具有良好的发展前景。本实验采用超声辅助提取法从干燥白芨块茎中提取白芨多糖,利用单因素实验和正交实验法寻求较适提取条件并进行多糖结构鉴定,对比分析传统水提法和超声提取法制得的白芨多糖的体外抗氧化能力。根据数据可得较适提取工艺条件院超声功率为400 W,料液比为1:35 g/mL,超声时间为50 min,此条件下白芨多糖得率达到8.18%依0.04%。超声辅助法制得的白芨多糖对DPPH窑、窑OH 的清除率明显高于传统水提法制得的白芨多糖。两种提取方法制得的多糖对ABTS+窑清除率无明显差异,均无O2-窑清除能力。

关键词: 白芨多糖, 超声辅助提取, 抗氧化

Abstract: Bletilla striata polysaccharide has many applications and development prospects in medicine and food science. In this experiment, the polysaccharides from Bletilla striata were extracted by ultrasonic-assisted extraction method. The single factor experiment and orthogonal experiment were used to find the best conditions and then identified its structure. The traditional water extraction method and ultrasonic extraction method were used to compare antioxidant activity of Bletilla striata polysaccharide. According to the data, the optimal extraction process conditions were obtained: the ultrasonic power was 400 W, the ratio of material to liquid was 1:35 g/mL, and the ultrasonic time was 50 min. The yield of the polysaccharide was 8.18% + 0.04% under this condition. The scavenging rate of DPPH?and?OH of Bletilla striata polysaccharide by ultrasonic-assisted extraction method was higher than
by traditional water extraction method. The polysaccharides obtained by the two extraction methods showed no significant difference in ABTS+?clearance rate, and none of them had O2-?clearing ability.

Key words: Bletilla striata polysaccharide, Ultrasonic assisted extraction, antioxidant

中图分类号: 

  • TQ461