The spatial distribution and migration characteristics of As (arsenic) in soil–tea tree of selenium-enriched tea garden were investigated by microwave digestion-inductively coupled plasma mass spectrometry. The results showed that the average soil As content in the sample area was No.4 > No.2 > No.1 > No.3, and the highest value of As content in tea garden soil was 3.3337 mg/kg, which met the soil environmental quality standard. The distribution order of As content in tea plant from high to low was fibrous root > main root ≈ main stem > old leaf > tea leaf; the As enrichment coefficient of tea plant to soil was fibrous root > main root > main stem ≈ old leaf > lateral stem > new leaf. When the tea plant was polluted by arsenic, its root had buffer and barrier effect on preventing arsenic from migrating upward. The migration ability of main stem ≈ old leaf > lateral stem > new leaf, which means that the migration ability of main stem and old leaf was stronger. The As content of tea ranged from 0.0448 to 0.106 mg/kg, which was lower than the limit value. The daily intake of As content accounted for 0.09%~0.25% of ADI, so the health risk of drinking tea was low.