By using the research methods of literature review and field interview, this paper deeply analyzes the physical education internship under the accreditation of teacher-training majors in colleges and universities in China. By combining the development orientation of teacher-training majors, based on the development characteristics of physical education majors, and aiming at the actual dilemmas existing in physical education internship, such as ineffectiveness of internship links, lack of standardization of practice and study links, unclear training objectives, and lack of reasonable and effective quality monitoring and evaluation system, this paper proposes to optimize the development strategy of physical education internship under the accreditation of teacher-training majors, pay attention to the close connection between all links of education internship and the integration of theory and practice, ensure the effective development of education internship, strengthen the quality monitoring and evaluation system of education internship, establish a multi-faceted cooperation system, and jointly help improve education internship. The study aims provide reference for the reform of physical education internship under the accreditation of teachertraining majors.