According to the SITC double-digit classification standard, 61 categories of export products are selected, and the export comparative advantage dynamics of Anhui and Jiangsu are analyzed based on the revealed symmetric comparative advantage index(RSCA). From 2000 to 2019, most of the export products of the two provinces have no comparative advantage, and the distribution of comparative advantage of Anhui is more scattered than that of Jiangsu. In the past 20 years, the export comparative advantage of the two provinces has changed significantly, which is the result of cumulative small changes in each year. About 30% of the most dynamic products in the two provinces remain in a state of survival. Therefore, the two provinces should steady export growth, adjust the structure, focus on the main export products, and implement the integration of the Yangtze River Delta market, so as to enhance the comparative advantage and survival ability of export.