The important goal of talent training for application-oriented universities is to produce innovative and entrepreneurial talents that meet the needs of the society. It is very important to establish a precise and scientific evaluation system for application-oriented undergraduate students' innovation and entrepreneurship ability. From the perspective of OBE, the entropy method and TOPSIS method were comprehensively used to construct the evaluation system for the entrepreneurship and innovation ability of application-oriented undergraduate business administration students. And the relevant data of a university in Anhui Province from 2016 to 2020 were used for empirical analysis. The results show that: in the evaluation index system of entrepreneurship and innovation ability of applicationoriented undergraduate business administration students, the weights from high to low are the output of comprehensive ability, the output of entrepreneurial ability and the output of innovation ability. By further improving the use of OBE concept, innovating the talent training mode, strengthening the innovation and innovation education reform, and striving to improve the innovation and innovation ability of application-oriented undergraduate business administration students, this paper provides reference and basis for promoting the high-quality development of application-oriented undergraduate universities.