The outbreak of COVID-19 caused by the new type of coronavirus has a great influence on the normal production and life order of the people, and has also caused the whole society to attach importance to the viral infectious diseases. From the characteristics of the new coronavirus, the supplement and adjustment of microbiology course content, the application of teaching mode of combining network teaching with constructive teaching method,the building of good hygiene habits, the concept of harmony with animals and the cultivation of social responsibility,this paper expounds the thinking on reform in microbiology course in the current severe situation of prevention and control of COVID-19.