Screening and Identification of Mycorrhizal Fungi against Fusarium oxysporum

ZHANG Yan; YUAN Quan; LIU Li-li; FANG Ming-ming; XU De-cong

doi:10.12152/j.issn.1672-2868.2019.03.011

Journal of Chaohu University >

2019 , Vol. 21 >Issue 3: 78 - 88

DOI: https://doi.org/10.12152/j.issn.1672-2868.2019.03.011

Screening and Identification of Mycorrhizal Fungi against Fusarium oxysporum

ZHANG Yan ,
YUAN Quan ,
LIU Li-li ,
FANG Ming-ming ,
XU De-cong

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School of Life Sciences, Hefei Normal University, Hefei Anhui 230601

Zhang Yan

Received date: 2019-04-22

Online published: 2019-05-25

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Abstract

Abstract: Fusarium oxysporum is the pathogen of blueberry root rot. The symbiotic mycorrhizal fungi of blueberry root play a role in the growth and development of blueberry and can antagonize harmful microorganisms. In this study, mycorrhizal fungi were isolated from blueberry roots in different areas of Anhui Province. The strains antagonistic to Fusarium oxysporum were screened by bacteriostatic experiment to explore the biological characteristics of antagonistic bacteria and optimize the culture conditions. The results showed that the root infection of blueberry was quite different in different areas of Anhui province. A total of 17 strains of endophytic mycorrhizal fungi were isolated, of which 6 strains were mycorrhizal fungi with antagonistic effect of Fusarium oxysporum.Trichoderma spinosum Hfsu02 had the best antagonistic effect. Under the condition of shaking flask culture in basic
culture medium, the optimum growth temperature was 25益, the optimum pH was 4, the logarithmic growth period was 12 ~112 h, and the highest yield of dry weight of mycelium was 2.76 g/L. The screening and culture optimization of Trichoderma spinosum Hfsu02 provide theoretical basis for the control of blueberry root rot, and have important guiding significance in the biological control of blueberry root rot.

Key words： Key words: Mycorrhizal Fungi; Fusarium oxysporum; Trichoderma spinosum; biological control

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ZHANG Yan , YUAN Quan , LIU Li-li , FANG Ming-ming , XU De-cong . Screening and Identification of Mycorrhizal Fungi against Fusarium oxysporum[J]. Journal of Chaohu University, 2019 , 21(3) : 78 -88 . DOI: 10.12152/j.issn.1672-2868.2019.03.011

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