拮抗尖孢镰刀菌的蓝莓菌根真菌的筛选和鉴定

张雁; 袁泉; 刘莉莉; 方明明; 徐德聪

doi:10.12152/j.issn.1672-2868.2019.03.011

巢湖学院学报 >

2019 , Vol. 21 >Issue 3: 78 - 88

DOI: https://doi.org/10.12152/j.issn.1672-2868.2019.03.011

生命与环境科学

拮抗尖孢镰刀菌的蓝莓菌根真菌的筛选和鉴定

张雁 ,
袁泉 ,
刘莉莉 ,
方明明 ,
徐德聪

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合肥师范学院生命科学学院，安徽合肥 230601

作者简介:张雁(1984-)，男，安徽巢湖人，合肥师范学院生命科学学院讲师，主要从事微生物代谢研究。

收稿日期: 2019-04-22

网络出版日期: 2019-05-25

基金资助

基金项目：安徽省高校自然科学基金重点项目（项目编号：KJ2018A0494）；安徽省自然科学基金面上项目（项目编号：1608085MC64）；国家大学生创新训练项目（项目编号：201914098007）

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Screening and Identification of Mycorrhizal Fungi against Fusarium oxysporum

ZHANG Yan ,
YUAN Quan ,
LIU Li-li ,
FANG Ming-ming ,
XU De-cong

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School of Life Sciences, Hefei Normal University, Hefei Anhui 230601

Zhang Yan

Received date: 2019-04-22

Online published: 2019-05-25

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摘要

摘要：尖孢镰刀菌（Fusarium oxysporum）是蓝莓根腐病的病原菌。蓝莓根际共生菌根真菌对蓝莓的生长发育有一定的促进作用，并能拮抗有害微生物。本研究从安徽不同地区的蓝莓根系中分离得到菌根真菌。通过抑菌试验，筛选出对尖孢镰刀菌具有拮抗作用的菌株，探讨了拮抗细菌的生物学特性，并对其培养条件进行了优化。结果表明，安徽省不同地区蓝莓根系中菌根真菌侵染有较大差异；共分离到17株内生菌根真菌，其中6 株为对尖孢镰刀菌有拮抗作用的菌根真菌。拮抗作用最强的是棘孢木霉fsu02（Trichoderma spinosum Hfsu02).在基础培养基摇瓶培养条件下，其最适生长温度为25益，最适pH 为 4，12~112 h 为对数生长期，菌丝干重最高为2.76 g/L，该菌株的筛选和鉴定为蓝莓根腐病的防治提供了理论依据，对蓝莓根腐病的生物防治具有重要的指导意义。

关键词： 关键词：菌根真菌; 尖孢镰刀菌; 棘孢木霉; 生物防治

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张雁 , 袁泉 , 刘莉莉 , 方明明 , 徐德聪 . 拮抗尖孢镰刀菌的蓝莓菌根真菌的筛选和鉴定[J]. 巢湖学院学报, 2019 , 21(3) : 78 -88 . DOI: 10.12152/j.issn.1672-2868.2019.03.011

Abstract

Abstract: Fusarium oxysporum is the pathogen of blueberry root rot. The symbiotic mycorrhizal fungi of blueberry root play a role in the growth and development of blueberry and can antagonize harmful microorganisms. In this study, mycorrhizal fungi were isolated from blueberry roots in different areas of Anhui Province. The strains antagonistic to Fusarium oxysporum were screened by bacteriostatic experiment to explore the biological characteristics of antagonistic bacteria and optimize the culture conditions. The results showed that the root infection of blueberry was quite different in different areas of Anhui province. A total of 17 strains of endophytic mycorrhizal fungi were isolated, of which 6 strains were mycorrhizal fungi with antagonistic effect of Fusarium oxysporum.Trichoderma spinosum Hfsu02 had the best antagonistic effect. Under the condition of shaking flask culture in basic
culture medium, the optimum growth temperature was 25益, the optimum pH was 4, the logarithmic growth period was 12 ~112 h, and the highest yield of dry weight of mycelium was 2.76 g/L. The screening and culture optimization of Trichoderma spinosum Hfsu02 provide theoretical basis for the control of blueberry root rot, and have important guiding significance in the biological control of blueberry root rot.

Key words： Key words: Mycorrhizal Fungi; Fusarium oxysporum; Trichoderma spinosum; biological control

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